The Class I Histone Deacetylase Inhibitor MS-275 Prevents Pancreatic Beta Cell Death Induced by Palmitate

Figure 3

Impact of HDAC1 and HDAC3 silencing on apoptosis evoked by palmitate. (a) Western blotting analysis of HDAC1 and HDAC3 abundance and (b) measurement of apoptosis upon silencing of HDAC1 and HDAC3 in MIN6 cells. Cells were transfected with the siRNAs directed against either HDAC1 (siH1), HDAC3 (siH3), or GFP (ctrl). Palmitate was added 24 hrs after transfection. Nuclear proteins were prepared and pyknotic nuclei were counted 48 hrs later. The immunoblotting was achieved using the anti-HDAC1, anti-HDAC3, and anti-β-actin antibodies. The figure shows the result of a representative experiment out of three.

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