The Class I Histone Deacetylase Inhibitor MS-275 Prevents Pancreatic Beta Cell Death Induced by Palmitate

Figure 4

Effects of HDAC1 and HDAC3 silencing on the ER stress markers. The Atf3 and Chop level of MIN6 cells transfected with siH1, siH3, or control siRNAs (siGFP) was quantified by (a) quantitative PCR and (b) western blotting experiments. Cells were cultured with palmitate (filled bars) or BSA (open bars) 24 hrs after transfection. Total RNA and nuclear protein extracts were prepared 48 hrs later. The mRNA levels were normalized against the Rplp0 and the expression levels from cells cultured with BSA (−) and transfected with siGFP were set to 100%. Data are the mean of ± SEM of 3 independent experiments (; ). For western blotting analysis, the anti-Atf3, anti-Chop, and anti-Tbp as the control were used. The figure shows the result of a representative experiment out of three.

(a)

(b)