Downregulation of Bcl-2 Expression by miR-34a Mediates Palmitate-Induced Min6 Cells Apoptosis
Figure 4
Bcl-2 is functionally related to the effect of miR-34a in response to palmitate. (a) Min6 cells transfected with specific siRNAs against mouse Bcl-2. WB assay were performed to confirm the effect of siRNAs. α-Tubulin was used as loading control. (b) Min6 cells were transfected with specific siRNAs against mouse Bcl-2. The cells were cultured for 48 h in DMEM containing 1 mM palmitate. The number of apoptotic cells was evaluated by scoring cells displaying pycnotic nucleus and/or fragmented nucleus visualized with Hoechst 33342. (c) Reexpression of Bcl-2 with the expression plasmid pcDNA3-M-Bcl-2 in the miR-34a-overexpressing Min6 cells. WB assay was performed to confirm the effect of Bcl-2 overexpression. α-Tubulin was used as loading control. (d) Reexpression of Bcl-2 partially antagonized the miR-34a-induced cell apoptosis. For (b) and (c), data are means ± SD of triplicate assays in three independent experiments *.
