Chicken Embryos as a Potential New Model for Early Onset Type I Diabetes
Figure 4
The STZ-induced diabetic retina exhibits changed physiology. (a1-a3) ERGs were recorded from E20 embryos. (a1) Representative ERG waveforms from a control and a STZ-injected embryo. (a2) The amplitudes of a- and b-waves from STZ-injected embryos are significantly lower than the ones recorded from controls. (a3) The implicit time of both a- and b-waves from STZ-injected embryos are delayed, but there is a significant increase of the b-wave implicit time from STZ-injected embryos compared to the control. (b1-b2) Cone photoreceptors from control and STZ-injected embryonic retinas were dissociated and cultured at E18. The next day, patch-clamp recordings of L-VGCCs were carried out. (b1) The average data of voltage (mV) current density (pA/pF) relationship from the control and STZ-diabetic photoreceptors are shown. (b2) At both −40 mV and 0 mV, the L-VGCC current densities are significantly lower from STZ-diabetic photoreceptors compared to the control. (c) The STZ-injected diabetic retina has a significant decrease in the protein level of the L-VGCCα1D subunit compared to the control. Total ERK serves as a loading control. .
