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Journal of Diabetes Research
Volume 2014, Article ID 376570, 10 pages
http://dx.doi.org/10.1155/2014/376570
Research Article

Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells

1Department of Cardiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Xiamen 361003, China
2Department of Cardiology, Chaoyang Hospital, Capital Medical University, 8th Gongtinanlu Road, Chaoyang District, Beijing 100020, China

Received 6 April 2014; Accepted 16 April 2014; Published 4 June 2014

Academic Editor: Nikolaos Papanas

Copyright © 2014 Jieqiong Zhou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to investigate the effects of simvastatin on insulin secretion in mouse MIN6 cells and the possible mechanism. MIN6 cells were, respectively, treated with 0 μM, 2 μM, 5 μM, and 10 μM simvastatin for 48 h. Radio immunoassay was performed to measure the effect of simvastatin on insulin secretion in MIN6 cells. Luciferase method was used to examine the content of ATP in MIN6 cells. Real-time PCR and western blotting were performed to measure the mRNA and protein levels of inward rectifier potassium channel 6.2 (Kir6.2), voltage-dependent calcium channel 1.2 , and glucose transporter-2 (GLUT2), respectively. ATP-sensitive potassium current and L-type calcium current were recorded by whole-cell patch-clamp technique. The results showed that high concentrations of simvastatin (5 μM and 10 μM) significantly reduced the synthesis and secretion of insulin compared to control groups in MIN6 cells . ATP content in simvastatin-treated cells was lower than in control cells . Compared with control group, the mRNA and protein expression of Kir6.2 increased with treatment of simvastatin , and mRNA and protein expression of and GLUT2 decreased in response to simvastatin . Moreover, simvastatin increased the ATP-sensitive potassium current and reduced the L-type calcium current. These results suggest that simvastatin inhibits the synthesis and secretion of insulin through a reduction in saccharometabolism in MIN6 cells.