BlockingαVβ3 Integrin Ligand Occupancy Inhibits the Progression of Albuminuria in Diabetic Rats

Figure 4

Biochemical analysis of kidney lysates. Kidney lysates from several rats in each group (-9) were prepared and equal amounts of total protein were separated by SDS-PAGE prior to immunoblotting (IB) with either an anti-TGF-β, anti-type IV collagen, or anti-TS-1 antibody. To demonstrate that any difference in protein levels was not due to a difference in the total amount of lysate, these extracts were also immunoblotted with an anti-actin antibody. The results from representative lysates of each group are shown. For each treatment group the protein band intensities from all the rats in the group were quantified using scanning densitometry. The arbitrary scanning units (mean ± SEM) are shown in Table 2.