Generating and Reversing Chronic Wounds in Diabetic Mice by Manipulating Wound Redox Parameters

Figure 4

Treating chronic wounds with AOA leads to proper healing and reduced oxidative stress. (a) Chronic wounds of db/db mice treated with IAE and then AOA, NAC, and α-toc, show a faster rate of wound closure. Wound areas were traced and analyzed using Image J. (b) Percent open wound area in db/db wounds treated with IAE and then AOA compared to db/db wounds treated with IAE alone. AOA significantly accelerated wound closure. (c) Weight loss was significantly higher in db/db mice treated with IAE alone than when treated with IAE and then AOA. (d) The levels of SOD in db/db wounds treated with PBS were significantly higher than db/db wounds treated with IAE suggesting that the latter have reduced dismutation of radicals and accumulation of reactive radicals. AOA treatment of chronic wounds significantly increased SOD activity 10 days after treatment suggesting increased dismutation of radicals. (e) Increases in H₂O₂ levels (examined as described in Figure 1) in db/db wounds treated with AOA were stabilized in comparison to the increasing stress in the non-AOA treated wound. (f) Catalase activity was significantly increased in PBS treated wounds in the first 15 days as compared to the IAE treated wounds. AOA treatment of chronic db/db wounds significantly increased catalase activity by 20 days of treatment. (g) GPx in the db/db mice treated with PBS was significantly higher than the db/db mice treated with IAE. Enzyme activity in the db/db chronic wounds treated with AOA was significantly higher than db/db mice with only IAE. The overall effect was stabilized levels in H₂O₂. All data are mean ± SD. , . or 7 for each of the studies unless indicated differently.

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