Generating and Reversing Chronic Wounds in Diabetic Mice by Manipulating Wound Redox Parameters

Figure 6

Histological evaluation of normal, chronic, and treated wounds: (a) H&E-stained section of day 64 chronic wound in db/db mice treated with IAE. (b) H&E-stained sections of representative animals to illustrate the histology at wound closure of db/db wounds from a nontreated animal (panel 1), an animal treated with IAE, and then with AOA α-toc (panel 2), an animal treated with IAE, and then with AOA NAC (panel 3) and an animal treated with IAE and then both AOA (panel 4). Scale bar 100 µm. (c) Representative Masson-trichrome staining (blue color) illustrating loss of collagen deposition in nontreated db/db mice (panel 1). A decrease in collagen deposition was seen in chronic wounds treated with AOA individually (panels 2 and 3). Chronic wounds treated with both AOA had a significant increase in collagen deposition and fibril formation (panel 4). Second Harmonic Generated Imaging (SHIM) shows abnormalities in collagen bundles in the nontreated db/db wounds whereas no clear fibers are seen in individual AOA treated wounds. In wounds with both AOA, fibers are well formed and appear more mature. For SHIM the scale bar is 10 µm. (d) Keratin 10 illustrates the presence of the epithelial cytoskeleton in the terminally differentiating epithelial cells found in the suprabasal layer. The epithelium is much more mature in the wounds treated with both AOA. Scale bar is 100 µm.

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