Role of MicroRNAs in Islet Beta-Cell Compensation and Failure during Diabetes

Figure 2

Identification of miRNAs differentially expressed in MIN6 cells cultured with human native and oxidized LDL. The expression of the indicated miRNAs was measured by quantitative RT-PCR in MIN6 cells that were cultured with vehicle, 2 mmol/L of oxidized LDL (oxLDL), and native LDL (NaLDL), plus or minus 1 mmol/L HDL-cholesterol for 72 hrs. Human plasma LDL and HDL fractions were isolated by sequential ultracentrifugation (LDL density, 1.063) as described [95]. Oxidation of LDL particles was done by incubation of 1 mg LDL protein/mL PBS with 5 μmol/L CuSO4 at 37°C for 6–8 h [95]. The oxidation reaction was verified as previously described by determining the lipid peroxide content [95]. The results are expressed as fold changes and correspond to the mean ± SD.