Research Article
No Evidence of Viral Transmission following Long-Term Implantation of Agarose Encapsulated Porcine Islets in Diabetic Dogs
Table 1
Preimplant microbiological and vial screening of porcine islet macrobeads.
| | Test description | Testing facility | Methodology |
| | Total product bioburden testing | Microtest Laboratories, Inc. | ANSI/AAMI/ISO 11737-1 USP membrane filtration | | Salmonella sp. screening | Microtest Laboratories, Inc. | USP | | Sterility | Microtest Laboratories, Inc. | USP membrane filtration | | Mycoplasma | Microtest Laboratories, Inc. | Direct cultivation methods
Cell culture methods | | Bacterial endotoxin | Microtest Laboratories, Inc. | Kinetic chromogenic | | Porcine reproductive and respiratory syndrome virus (PRRSV) | VDL | RT-PCR | | Swine influenza virus (SIV) | VDL | RT-PCR | | Porcine endogenous retrovirus (PERV) | VDL | RT-PCR | | Porcine enterovirus (PEV) | VDL | RT-PCR | | Porcine respiratory corona virus (PRCV) | VDL | RT-PCR (Qiagen 283615) | | Transmissible gastroenteritis virus (TGEV) | VDL | RT-PCR (Qiagen 283615) | | Porcine circovirus types 1 and 2 (PCV) | VDL | Multiplex PCR | | Porcine lymphotropic herpes virus type-1 (PLHV-1) | VDL | PCR | | Swine hemagglutinating encephalomyelitis virus (sHEV) | VDL | RT-PCR | | Porcine parvovirus (PPV) | VDL | PCR | | Porcine cytomegalovirus (PCMV) | VDL | PCR | | Mycoplasma hyopneumoniae | VDL | PCR | | Pseudorabies virus (PRV) | VDL | Virus isolation | | Encephalomyocarditis virus (EMCV) | VDL | Virus isolation | | Rotavirus (type A) | VDL | Antigen capture ELISA | | Chlamydia spp. | VDL | Antigen capture ELISA |
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