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Journal of Diabetes Research
Volume 2014, Article ID 745419, 12 pages
http://dx.doi.org/10.1155/2014/745419
Research Article

Evaluation of Hypoglycemic Efficacy of Tangningtongluo Formula, a Traditional Chinese Miao Medicine, in Two Rodent Animal Models

1Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Ministry of Education, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China
2Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China
3Pharmaceutical College, Harbin University of Commerce, Harbin, Heilongjiang 150076, China
4Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China

Received 1 July 2014; Revised 4 September 2014; Accepted 30 October 2014; Published 5 November 2014

Academic Editor: Toshiyasu Sasaoka

Copyright © 2014 Long Cheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Traditional Chinese medicines largely lack adequate and scientifically rigorous evidence regarding efficacy and functional mechanisms. The present study was aimed to confirm the hypoglycemic effect of Tangningtongluo (TNTL) formula, a traditional Chinese Miao medicine, in two animal models: high-fat diet and streptozotocin- (STZ-) induced diabetic rats and C57BL/KsJ-db/db diabetic mice. After 4 weeks, TNTL intervention in STZ-induced diabetic rats yielded in significant improvement on the glucose tolerance test. Moreover, the islet histopathology showed that oral TNTL reduced the severity of islet necrosis in pancreases tissue. Compared with diabetic controls, a 12-week TNTL treatment regimen (dosages = 0.9, 1.8, and 3.6 g/kg) in db/db mice significantly decreased fasting glucose and HbA1c. Additionally, oral glucose tolerance in TNTL-treated mice improved significantly, compared with diabetic mice receiving metformin. Finally, tissue histopathology and biochemical index evaluations revealed significant improvement in TNTL-treated mice. Taken together, our results show that TNTL exerted a strong hypoglycemic effect in two diabetic rodent animal models, preserving -cells in the pancreas islet and reducing the risk of diabetic retinopathy and nephropathy.