Hyperglycemia Inhibits Complement-Mediated Immunological Control of S. aureus in a Rat Model of Peritonitis

Figure 5

Neutrophil migration into the peritoneum. (a) Leukocytes in the peritoneal fluid wash were quantitated by microscopy of Wright strained cytospin slides. (b) Myeloperoxidase activity (MPO) in the peritoneal fluid wash measured using TMB. (c) Free DNA in the peritoneal fluid wash measured by spectrophotometry using PicoGreen. Graphed data shows means of results for peritoneal samples from the animals in each treatment group. Error bars denote standard error of the means. S. aureus inoculation dose ranging experiments with peritoneal wash performed at 24 hours after infection. (d) Leukocyte enumeration of peritoneal wash fluid performed by microscopy of Wright-stained cytospin slides. (e) S. aureus recovered from peritoneal wash fluid assayed by colony counting.

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