Journal of Diabetes Research / 2014 / Article / Fig 2

Research Article

Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions

Figure 2

Effects of EMD and/or adiponectin (3 μg/mL) on wound closure over 4 d (a). Untreated cells were used as control. Mean ± SEM ( ); *significantly ( ) different from cells treated with EMD, either alone or with adiponectin; #significantly ( ) different from all other groups. Effects of EMD and/or adiponectin (1 μg/mL) on the mRNA expression of Ki67 at 1 d and 3 d (b). Untreated cells were used as control. Mean ± SEM ( ); *significant ( ) difference between groups. Effects of EMD on SMAD1/5/8 nuclear translocation in the presence and absence of adiponectin (1 μg/mL) at 60 min, as analyzed by immunofluorescence (c–f). Untreated cells served as control. Experiments were performed in triplicate and repeated twice. Images from one representative donor are shown.
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(a)
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(b)
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(c)
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(d)
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(e)
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(f)

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