JNK3 Is Required for the Cytoprotective Effect of Exendin 4
Figure 2
Impact of the JNK3 silencing on the protective effects of exendin 4. INS-1E cells were transfected with the siRNA duplex directed against JNK3 (siJNK3) or control siRNA (siGFP, Ctrl). (a) For western blotting analysis of the JNK3 level, total proteins were prepared 48 hr after transfection. Immunoblotting was done using the anti-JNK3, anti-JNK2, and anti-α-tubulin antibodies (b) for scoring death; the cells were preincubated 24 hr after transfection with 50 nM exendin 4 for 8 hr. The rate of apoptosis was scored by counting pycnotic nuclei in INS-1E cells exposed for 16 hr to the cocktail of cytokines including 10 ng/mL IL-1β, 15 ng/mL TNFα, and 150 ng/mL IFNγ. Results are expressed as mean ± SEM of 3 independent experiments. ; .
