P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells
Figure 3
Effect of preincubation with MAPK inhibitors on GLU+PA-induced apoptosis of INS-1 cells. (a) INS-1 cells were grown on 60 mm coverslips and preincubated with MAPK inhibitors (JNK: SP60015, P38: SB 203580, ERK: PD98059) before GLU+PA treatment. Cell morphology was examined and images were acquired by phase-contrast microscopy. (b) The cells were fixed and stained with Hoechst 33342 and at 96 h after GLU+PA treatment. The apoptotic percentages were calculated. **Comparing to control (only DMSO was added into GLU+PA-treated cells), (ANOVA).
