Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAK/STAT Pathway in Human Pancreatic β Cells

Figure 6

Effects of STAT3 knockdown on IL-6 + Dx-induced mRNA expression of REG Iα and REG Iβ in human β cells. 1.1B4 cells were transfected with scrambled siRNA control or STAT3-specific siRNA and then stimulated without or with IL-6 (20 ng/mL) + Dx (100 nM). The mRNA levels of (a) STAT3, (b) REG Iα, and (c) REG Iβ were measured by real-time RT-PCR using β-actin as an endogenous control. Data are represented as the ratio of the number of target mRNA copies to the number of β-actin mRNA copies and expressed as means ± SE for each group ((a) , (b) -4, (c) ). ; .

(a)

(b)

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