Therapeutic Benefit of Extended Thymosin β4 Treatment Is Independent of Blood Glucose Level in Mice with Diabetic Peripheral Neuropathy
Effect of Tβ4 on MBP, NF-H, and morphometric change of myelinated sciatic nerves. Panels (a) to (c) show semithin toluidine blue-stained cross sections of sciatic nerves from a representative nondiabetic mouse (a), diabetic mouse treated with saline (b), and diabetic mouse treated with Tβ4 (c) for 16 weeks. Panels (d) to (f) are double immunofluorescent images showing MBP and NF-H immunoreactive sciatic nerves of a representative nondiabetic mouse (d), diabetic mouse treated with saline (e), and diabetic mouse treated with Tβ4 (f) for 16 weeks. Panels (g) and (h) show quantitative data of the percentage of MBP (g) and NF-H (h) immunoreactive area. Bars in μm and μm. versus the nondiabetic group (DM) and versus diabetic mice treated with saline (DB). /group.