Proteases and Protease Inhibitors of Urinary Extracellular Vesicles in Diabetic Nephropathy

Figure 5

Zymography SDS-PAGE of vesicle-associated gelatinases. One μg of protein per each pool (Bradford assay) was loaded in each lane. Gel was incubated at 37°C for 16 hours. Try: Trypsin (50 ng), H: healthy, N: normoalbuminuric, Mi: microalbuminuric, and Ma: macroalbuminuric pools.