Effects of High Glucose Levels and Glycated Serum on GIP Responsiveness in the Pancreatic Beta Cell Line HIT-T15

Figure 2

Treatments with GS and HG do not alter GIP-induced phosphorylation of p42/44 ERK (Thr202/Tyr204). After 5-day treatment in the presence of standard medium (CTR) or high glucose concentration (11.1 mmol/L) (HG) in presence of glycated serum (GS), HIT-T15 cells were incubated for 2 hours in serum-free medium and then stimulated with 100 nmol/L GIP for 5 min (dark bars). (a) Representative western blot analysis. (b), (c) Quantification of densitometries of western blot bands. Data were expressed as mean ± SE of fold induction relative to GAPDH (). , , and versus absence of GIP.

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