Aberrant Phenotype in Human Endothelial Cells of Diabetic Origin: Implications for Saphenous Vein Graft Failure?

Figure 2

SV-EC migration. Confluent monolayers were quiesced and then wounded to create a cell-free area. Cells were cultured in medium containing 1–20% FCS and images taken at 0 and 24 h. The number of cells migrated past the wound edge were counted. (a) Concentration response to FCS, expressed relative to migration observed in 1% FCS (, , , ns = not significant relative to 1% migration). (b) Summary data of migration in media containing 10% FCS (, ). (c) Representative images, scale bar = 100 μm. Cells can clearly be seen migrating past the wound edge at 24 h.

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