Nutrient Induced Type 2 and Chemical Induced Type 1 Experimental Diabetes Differently Modulate Gastric GLP-1 Receptor Expression

Figure 2

Opposite expression of GLP-1R in glandular stomach of PO and SPD rats after diabetes induction. (a) RT (q)PCR analysis demonstrates that GLP-1R mRNA levels were significantly increased () or decreased () in diabetic PO and SPD rats (black columns), respectively, as compared to normoglycemic untreated animals (white columns). (b, c, d) Semiquantitative immunohistomorphological analysis of glandular GLP-1R expression of diabetic and control PO rats. (b) Significantly increased stain intensity of GLP-1R immunoreactive cells in neck area of principal glands of diabetic (black columns) compared with control (white columns) PO rats. The arbitrary lines presented in illustrations (c) and (d) and (f) and (g) divide the entire gastric glands into three areas named neck, mid, and bottom areas. The neck area includes very narrow isthmus and neck areas of gastric glands; mid area, the upper area of gastric gland base; bottom area, the deep area of gastric gland base. (c, d) GLP-1R immunostained longitudinal sections of glandular stomach mucosa (magnification ×10) demonstrate general increase of stain intensity of GLP-1R immunoreactive glandular cells (brown color) most prominently in the neck of principal glands of diabetic (d) compared with control (c) PO rats. (e, f, g) Semiquantitative immunohistomorphological analysis of glandular GLP-1R expression in diabetic and control SPD rats. (e) Significantly decreased stain intensity of GLP-1R immunoreactive cells in neck () and mid areas () of principal glands of diabetic (black columns) compared with control (white columns) SPD rats. (f, g) GLP-1R immunostained longitudinal sections of glandular stomach mucosa (magnification ×10) demonstrate a general decrease stain intensity (most prominent in the neck area) of GLP-1R immunoreactive glandular cells (brown color) in diabetic (g) compared with control (f) SPD rats. Data are presented as mean ± SD. Bar = 100 μm.