Diabetic Nephropathy Induced by Increased Ace Gene Dosage Is Associated with High Renal Levels of Angiotensin (1–7) and Bradykinin

Table 2

Quantification of Ang I, Ang II, and Ang-(1–7) levels in kidney tissue of CT and D mice harboring 1 or 3 copies of the Ace gene at twelve weeks of experimental period assessed by HPLC.

Peptide concentration (pmol/g)	1 copy		3 copies
	Control	Diabetic	Control	Diabetic
Ang I	12.4 ± 0.3	10.4 ± 0.5	15.9 ± 0.9	7.2 ± 0.
Ang II	106.9 ± 9.5	85.1 ± 6.	172.6 ± 6.	126.5 ± 2.
Ang-(1–7)	174.9 ± 8.2	283.7 ± 34.	474.6 ± 5.	556.8 ± 10.

Values represent mean ± S.E.M. Two-way ANOVA showed : diabetic versus control. : 3 copies control group versus 1 copy control group. : 3 copies diabetic group versus 1 copy diabetic group.