Polysaccharides from Enteromorpha prolifera Improve Glucose Metabolism in Diabetic Rats

Figure 4

The effect of PEP on the histopathological structure of the pancreas in DM rats (IHC). A DM model was established by feeding rats a high-fat diet and injecting them with a low dose of STZ. After the model was established, PEP (0, 150, 300, and 600 mg/kg) was administered intragastrically as an intervention for 28 d ( rats). The pancreatic tissues were collected, embedded, and sectioned, and a specific antibody against insulin/proinsulin was utilized to observe the residual β-cells in islets by using immunohistochemical method (IHC). (A) control: control group; (B) model: model group; (C) metformin HC: metformin HC group; (D) 150 mg/kg: low-dose PEP group; (E) 300 mg/kg: medium-dose PEP group; (F) 600 mg/kg: high-dose PEP group. The arrow indicates islet tissue. (a) shows the histopathological structure of the pancreas in DM rats by IHC; (b) shows the mean integrated optical density of β-cells in islets in DM rats using the Image-Pro Plus 4.5 image analysis software.

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