Journal of Diabetes Research

Research Article

An Altered Pattern of Myocardial Histopathological and Molecular Changes Underlies the Different Characteristics of Type-1 and Type-2 Diabetic Cardiac Dysfunction

Table 1

Identification numbers of TaqMan Gene Expression Assays and sequence for the forward (F) and reverse (R) primers (from 5′ to 3′) and Universal Probe Library (UPL) probes used in quantitative real time PCR.
Gene targetTaqMan Gene Expression Assay ID
CatalaseRn00560930_m1
GAPDHRn01775763_g1
GSRRn01482159_m1
SOD2Rn00690587_g1
ThioredoxinRn00587437_m1
Gene targetPrimer sequenceUPL probes
ANFF: 5′-CAACACAGATCTGATGGATTTCA-3′   
R: 5′-CGCTTCATCGGTCTGCTC-3′		65
α-MHCF: 5′-GGAGGTGGAGAAGCTGGAA-3′  
R: 5′-ATCTTGCCCTCCTCATGCT-3′		65
β-actinF: 5′-CTAAGGCCAACCGTGAAAAG-3′  
R: 5′-TACATGGCTGGGGTGTTGA-3′		115
β-MHCF: 5′-GCTGCAGAAGAAGCTCAAAGA-3′  
R: 5′-GCAGCTTCTCCACCTTGG-3′		65
Caspase-12F: 5′-TGGATACTCAGTGGTGATAAAGGA-3′  
R: 5′-ACGGCCAGCAAACTTCATTA-3′		94
Collagen-1F: 5′-CTGGCAACCTCAAGAAGTCC-3′  
R: 5′-CAAGTTCCGGTGTGACTCG-3′		65
Collagen-3F: 5′-CCTGTTGGTCCATCTGGAAA-3′  
R: 5′-GACCTTGGGGACCAGGAG-3′		82
c-fosF: 5′-CCTGCAAGATCCCCAATG-3′  
R: 5′-AGTCAAGTCCAGGGAGGTCA-3′		115
c-junF: 5′-TACCGGCCAGCAACT TTC-3′  
R: 5′-TAGGCGCAGAAGAGGTTTTG-3′		115
eNOSF: 5′-TGACCCTCACCGATACAACA-3′  
R: 5′-CGGGTGTCTAGATCCATGC-3′		5
ET-1F: 5′-TGTCTACTTCTGCCACCTGGA-3′  
R: 5′-CCTAGTCCATACGGGACGAC-3′		115
GAPDHF: 5′-AGCTGGTCATCAATGGGAAA-3′  
R: 5′-CGGCAGGTCCTTCTCTATCA-3′		9
SOD1F: 5′-GGTCCAGCGGATGAAGAG-3′  
R: 5′-GGACACATTGGCCACACC-3′		5
Atrial natriuretic factor (ANF), alpha-myosin heavy chain (α-MHC), beta-myosin heavy chain (β-MHC), endothelial nitric oxide synthase (eNOS), endothelin-1 (ET-1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glutathione reductase (GSR), and superoxide dismutase 1 (SOD1) and 2 (SOD2).