Research Article
Edible Bird’s Nest Prevents High Fat Diet-Induced Insulin Resistance in Rats
Table 2
Names, accession number, and primer sequences used in the study.
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Housekeeping genes. Normalization gene. Underlined sequences are left and right universal left and right sequences (tags). Internal control supplied by Beckman Coulter Inc (Miami, FL, USA) as part of the GeXP kit. RT conditions were 48°C for 1 min; 37°C for 5 min; 42°C for 60 min; 95°C for 5 min and then hold at 4°C. PCR conditions were initial denaturation at 95°C for 10 min, followed by two-step cycles of 94°C for 30 sec and 55°C for 30 sec, ending in a single extension cycle of 68°C for 1 min. |