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Journal of Diabetes Research
Volume 2015 (2015), Article ID 789359, 7 pages
http://dx.doi.org/10.1155/2015/789359
Research Article

Comparative Evaluation of Two Venous Sampling Techniques for the Assessment of Pancreatic Insulin and Zinc Release upon Glucose Challenge

1Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
2Animal Resources Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
3Departments of Internal Medicine and Clinical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
4Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA

Received 19 April 2015; Revised 11 July 2015; Accepted 12 July 2015

Academic Editor: Hiroshi Okamoto

Copyright © 2015 Anil Kumar Pillai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Advances in noninvasive imaging modalities have provided opportunities to study β cell function through imaging zinc release from insulin secreting β cells. Understanding the temporal secretory pattern of insulin and zinc corelease after a glucose challenge is essential for proper timing of administration of zinc sensing probes. Portal venous sampling is an essential part of pharmacological and nutritional studies in animal models. The purpose of this study was to compare two different percutaneous image-guided techniques: transhepatic ultrasound guided portal vein access and transsplenic fluoroscopy guided splenic vein access for ease of access, safety, and evaluation of temporal kinetics of insulin and zinc release into the venous effluent from the pancreas. Both techniques were safe, reproducible, and easy to perform. The mean time required to obtain desired catheter position for venous sampling was 15 minutes shorter using the transsplenic technique. A clear biphasic insulin release profile was observed in both techniques. Statistically higher insulin concentration but similar zinc release after a glucose challenge was observed from splenic vein samples, as compared to the ones from the portal vein. To our knowledge, this is the first report of percutaneous methods to assess zinc release kinetics from the porcine pancreas.