In Vitro and In Vivo Effects of Metformin on Osteopontin Expression in Mice Adipose-Derived Multipotent Stromal Cells and Adipose Tissue

Figure 9

The mean concentration of OPN protein in the control (CTRL) and experimental cultures (1 mM, 5 mM, and 10 mM metformin) determined in supernatants collected at specific time of cultures (-axis). The statistically significant decrease of OPN protein was shown at 48 h and 72 h after treatment with 5 mM and 10 mM and in all experimental groups, respectively. The statistically significant difference determined in relation to the results obtained for control group was indicated with asterisks (). The mean values of OPN concentration were calculated from the results obtained in two measurements.