Adipose Tissue-Derived Mesenchymal Stem Cells Exert In Vitro Immunomodulatory and Beta Cell Protective Functions in Streptozotocin-Induced Diabetic Mice Model

Figure 1

Proliferation index of splenocytes from normal (a) and diabetic (b) mice cocultured with AT-MSCs in presence of PHA as nonspecific stimulator. The resting splenocytes showed a sharp proliferation response in the presence of PHA. AT-MSCs caused cell death in some resting splenocytes in the absence of mitogen (proliferation index < 1.0). AT-MSCs could suppress normal and diabetic splenocytes proliferation in response to PHA in a dose-dependent manner, where greater concentration of AT-MSCs induced cell death in some splenocytes (proliferation index < 1.0). Each bar represents the average of five independent experiments. indicates significant difference between groups (). PHA: phytohemagglutinin; SP: splenocytes.

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