Inhibition of TNF-α Reverses the Pathological Resorption Pit Profile of Osteoclasts from Patients with Acute Charcot Osteoarthropathy
Osteoclast formation and resorption in Charcot patients, diabetic patients, and healthy control subjects in M-CSF + RANKL-treated cultures and in M-CSF + RANKL + anti-TNF-α-treated cultures. Representative images of TRAP-positive multinucleated cells formed on plastic (Olympus; original magnification ×100, scale bar = 200 μm) (a) and resorbed bovine bone discs (Olympus BX60; original magnification ×200) (c). The arrows denote some of the TRAP-positive multinucleated cells (a) and some of the resorption pits (c). Comparison of the number of TRAP-positive multinucleated cells (b), the area of resorption at the surface (d), and the area of resorption under the surface (e) of resorbed bone discs between M-CSF + RANKL-treated cultures (white bars) and M-CSF + RANKL + anti-TNF-α-treated cultures (grey bars). Significance assessed by Mann-Whitney test, levels of significance are demonstrated on the graphs; ; ; ns = nonsignificant ().
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