The PI3K/Akt Signaling Pathway Mediates the High Glucose-Induced Expression of Extracellular Matrix Molecules in Human Retinal Pigment Epithelial Cells
Immunofluorescence staining of fibronectin, collagen IV, and laminin in RPE cells. The expression of fibronectin, collagen IV, and laminin was evaluated by pretreatment with 20 μM LY294002 for 12 h and then incubation with 24 h in RPE cells under high glucose conditions. The RPE cells showed more intense staining under high glucose conditions compared with NG or . (a) Fibronectin; (b) collagen IV; (c) laminin. DAPI: blue.