The PI3K/Akt Signaling Pathway Mediates the High Glucose-Induced Expression of Extracellular Matrix Molecules in Human Retinal Pigment Epithelial Cells

Figure 7

Immunofluorescence staining of fibronectin, collagen IV, and laminin in RPE cells. The expression of fibronectin, collagen IV, and laminin was evaluated by pretreatment with 20 μM LY294002 for 12 h and then incubation with 24 h in RPE cells under high glucose conditions. The RPE cells showed more intense staining under high glucose conditions compared with NG or . (a) Fibronectin; (b) collagen IV; (c) laminin. DAPI: blue.

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