Cholesterol-Induced Hepatic Inflammation Does Not Underlie the Predisposition to Insulin Resistance in Dyslipidemic Female LDL Receptor Knockout Mice

Figure 2

Hepatic inflammation in Ldlr^−/− mice fed an HFC diet. (a) Representative pictures of frozen liver sections stained with CD68 were taken from WT and Ldlr^−/− mice fed a chow, high-fat (HF), or high-fat cholesterol (HFC) diet ( = 5-6). RNA was isolated from liver tissue and the expression of the proinflammatory genes Cd68 (b), Cd11b (c), Tnfa (d), and Mcp1 (e) was determined by real-time PCR and expressed as fold induction (). Data are expressed as means ± SEM for WT mice (white bars) and Ldlr^−/− mice (black bars). WT versus KO; ^# HF, HFC versus chow; , HF versus HFC.

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