Testicular Metabolic Reprogramming in Neonatal Streptozotocin-Induced Type 2 Diabetic Rats Impairs Glycolytic Flux and Promotes Glycogen Synthesis

Figure 2

Type 2 diabetes mellitus (T2DM) modulates the (a) mRNA, (b) protein levels, and (d) activity of glycolytic enzymes and transporters. (a) Pooled data of independent experiments, indicating the fold variation of glucose transporters (GLUT1, GLUT2, and GLUT3) and of phosphofructokinase 1 (PFK1) mRNA levels found in testicles of T2DM rats when compared with the control rats (dashed line). (b) Pooled data of independent experiments, indicating the fold variation of GLUT1, GLUT2, GLUT3, and PFK1 protein levels found in testicles of T2DM rats when compared with the control rats (dashed line). (c) Illustrative Western Blot experiment. (d) Pooled data of independent experiments, indicating the fold variation of PFK1, lactate dehydrogenase (LDH), and alanine transaminase (ALT) enzymatic activities in testicles of T2DM rats when compared with the control rats (dashed line). Results are expressed as mean ± SEM ( for each condition). *Significantly different relative to control ().

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