Testicular Metabolic Reprogramming in Neonatal Streptozotocin-Induced Type 2 Diabetic Rats Impairs Glycolytic Flux and Promotes Glycogen Synthesis

Table 1

Oligonucleotides and cycling conditions for PCR amplification of GLUT1, GLUT2, GLUT3, PFK1, LDHA, MCT4, ALT2, GYS1, PYGL, and 2MG.

Gene	Sequence (5′-3′)	AT (C°)	Accession number
GLUT1	Sense: TCCGGCGGGAGACGCATAGT	61	NM_138827.1
	Antisense: CCCGCATCATCTGCCGACCC
GLUT2	Sense: GGGTTCCTTCCAGTTCGGAT	60	NM_012879.2
	Antisense: TCGTATGTGCTGGTGTGACT
GLUT3	Sense: GCGCAGCCCTTCCGTTTTGC	63	NM_017102.2
	Antisense: CCCCTCGAAGGCCCGGGTAA
PFK1	Sense: GAGTGCTGACAAGCGGCGGT	61	NM_013190.4
	Antisense: GTGGCCCAGCACGGTCACTC
LDHA	Sense: GCGCAGCCCTTCCGTTTTGC	63	NM_017025.1
	Antisense: CCCCTCGAAGGCCCGGGTAA
ALT2	Sense: TGAGGTAATCCGAGCCAACA	60	NM_001012057.1
	Antisense: CACGTCCTCTCGGATACAGT
MCT4	Sense: ATGTGGGCATGGCGTGTGCC	66	NM_001013913.1
	Antisense: CCCAGCCATGGCAGCTCGAA
GYS1	Sense: CAGCTATGGGACACAGCCAA	60	NM_001109615.1
	Antisense: TTCGTCGGATGGTGGTCAAG
PYGL	Sense: CTCCCAATCAGCCAGACCTC	60	NM_022268.1
	Antisense: GGAAGGCTCCATGTTCCAGA
2MG	Sense: ATGAGTATGCCTGCCGTGTG	60	NM_012512.2
	Antisense: CAAACCTCCATGATGCTGCTTAC

Legend: AT: annealing temperature; ALT2: alanine aminotransferase 2; GLUT1: glucose transporter 1; GLUT2: glucose transporter 2; GLUT3: glucose transporter 3; GYS1: muscle glycogen synthase; LDHA: lactate dehydrogenase A; MCT4: monocarboxylate transporter 4; PFK1: phosphofructokinase 1; PYGL: liver-type glycogen phosphorylase; 2MG: beta 2 microglobulin.