JNK1 Deficient Insulin-Producing Cells Are Protected against Interleukin-1β-Induced Apoptosis Associated with Abrogated Myc Expression

Figure 5

JNK1 knockdown attenuates the regulation of Myc mRNA expression by IL-1β. Stable INS-1 cell lines expressing shRNA for JNK1, JNK2, JNK3, nonsense shRNA, or empty vector controls were exposed to 150 pg/mL IL-1β (closed bars) or vehicle (open bars) for 2, 6, and 12 h. Relative mRNA expression were measured using quantitative RT-PCR and normalized to the average of 18S and Hprt1. (a) Relative Jun mRNA expression, (b) relative Trp53 mRNA expression, (c) relative Junb mRNA expression, (d) relative Jund mRNA expression, and (e) relative Myc mRNA expression. Data are shown as means ± SEM of 3–5 independent experiments. , , and , comparing expression in the presence with expression in the absence of IL-1β, unless otherwise indicated.

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