ALDH2 Inhibition Potentiates High Glucose Stress-Induced Injury in Cultured Cardiomyocytes

Figure 5

Disulfiram (DSF) pretreatment and mitochondrial membrane potential. (a) Representative microscopic images of cells with JC-1 dye to measure mitochondrial membrane potential (). Increase in red to green florescence ratio indicates high membrane potential as shown in mannitol group, while increased green florescence indicates drop in mitochondrial . (b) DSF 10 μM treatment reduces mitochondrial when measured using JC-1 dye in cultured H9C2 cardiomyocytes subjected to high glucose stress (HG) compared to equimolar mannitol (Ctrl). The data expressed are mean ± SEM. = 4–6. G1, G2, G3, and G4 depict mannitol, high glucose stress, DSF + mannitol, and DSF + high glucose stress, respectively. versus G1, versus G2, and versus G3.

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