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Journal of Diabetes Research
Volume 2016, Article ID 1482194, 6 pages
Research Article

Vascular Endothelial Growth Factor Gene Polymorphism (rs2010963) and Its Receptor, Kinase Insert Domain-Containing Receptor Gene Polymorphism (rs2071559), and Markers of Carotid Atherosclerosis in Patients with Type 2 Diabetes Mellitus

1Institute of Oncology Ljubljana, Zaloška 2, Sl-1000 Ljubljana, Slovenia
2Institute of Histology and Embryology, Faculty of Medicine, University in Ljubljana, Vrazov trg 2, Sl-1000 Ljubljana, Slovenia
3General Hospital Rakičan, Ulica dr. Vrbnjaka 6, Sl-9000 Murska Sobota, Slovenia
4General Hospital Slovenj Gradec, Gosposvetska Cesta 1, Sl-2380 Slovenj Gradec, Slovenia

Received 24 August 2015; Revised 24 October 2015; Accepted 1 November 2015

Academic Editor: Ronald G. Tilton

Copyright © 2016 Sebastjan Merlo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. The current study was designed to reveal possible associations between the polymorphisms of the vascular endothelial growth factor (VEGF) gene (rs2010963) and its receptor, kinase insert domain-containing receptor (KDR) gene polymorphism (rs2071559), and markers of carotid atherosclerosis in patients with type 2 diabetes mellitus (T2DM). Patients and Methods. 595 T2DM subjects and 200 control subjects were enrolled. The carotid intima-media thickness (CIMT) and plaque characteristics (presence and structure) were assessed ultrasonographically. Biochemical analyses were performed using standard biochemical methods. Genotyping of VEGF/KDR polymorphisms (rs2010963, rs2071559) was performed using KASPar assays. Results. Genotype distributions and allele frequencies of the VEGF/KDR polymorphisms (rs2010963, rs2071559) were not statistically significantly different between diabetic patients and controls. In our study, we demonstrated an association between the rs2071559 of KDR and either CIMT or the sum of plaque thickness in subjects with T2DM. We did not, however, demonstrate any association between the tested polymorphism of VEGF (rs2010963) and either CIMT, the sum of plaque thickness, the number of involved segments, hsCRP, the presence of carotid plaques, or the presence of unstable carotid plaques. Conclusions. In the present study, we demonstrated minor effect of the rs2071559 of KDR on markers of carotid atherosclerosis in subjects with T2DM.