The Different Effects of Atorvastatin and Pravastatin on Cell Death and PARP Activity in Pancreatic NIT-1 Cells

Figure 2

Treatment of AS in ROS-induced necrotic cell death. (a) The sub-G1 cell fractions were significantly dose-dependent and increased after exposure to AS; PS did not change cell cycle parameters. (b) Lower-left quadrants: viable cells. Lower-right quadrants: early apoptosis. Upper-left quadrants: necrotic cells. Upper-right quadrants: nonviable late apoptotic cells. An increased necrotic proportion of NIT-1 cells (Annexin V⁻PI⁺ cells) was observed after treatment with AS; no effect was observed in PS-treated cells. (c) IL-6 is a highly reliable marker of necrosis. IL-6 secretions in NIT-1 cells treated with AS; no IL-6 secretion was observed in PS-treated cells. The value for the CON group was set at 1. (d) Cells treated with AS or PS for 48 h were incubated with CellROX and measured using flow cytometry. The AS treatment of NIT-1 cells induced a dose-dependent increase of ROS production (, compared to the untreated group, mean ± SD from three replicates).

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