Influence of Aluminium and EGCG on Fibrillation and Aggregation of Human Islet Amyloid Polypeptide

Figure 3

(a) ¹H NMR spectra of 10 mM EGCG without (bottom) and with (top) 4 mM Al(III). (b) The ¹H resonance integration at δ = 6.04 ppm of C-9 in 10 mM EGCG on the [Al(III) ]/[EGCG] ratio as the titration of Al(III) from 0 to 30 mM. The values were normalized to ¹H integration within 2.77 to 2.95 ppm of C-4. (c) Illustration of Al(EGCG)(H₂O)₂ complex 1 (AE-1). Red, gray, pink, and white balls represent oxygen, carbon, aluminum, and hydrogen atoms, respectively. C₉ and O₈ in A-ring of EGCG and two more O atoms from H₂O coordinate to the aluminum.

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