Influence of Aluminium and EGCG on Fibrillation and Aggregation of Human Islet Amyloid Polypeptide

Figure 4

Self-aggregation of hIAPP. (a) Fibrillation kinetics of 16 μM hIAPP in 20 mM PBS at pH 7.4 under 37°C monitored by ThT fluorescence. The values represent mean (). Solid lines represent the curves fitted using (1). (b) TEM image of 16 μM hIAPP incubated in 20 mM PBS for 2 h. (c) Contour plots of ¹H NMR spectra within chemical shift from 7.13 to 7.3 ppm for 100 μM hIAPP incubated in 6 mM PBS at pH 7.4 and 25°C. (d) Aggregation kinetics of integration resonance intensity of ¹H NMR spectra from 7.13 to 7.3 ppm for 100 μM hIAPP. Solid lines represent the curves fitted using (1). The integration values were normalized to the initial hIAPP alone data.

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