Influence of Aluminium and EGCG on Fibrillation and Aggregation of Human Islet Amyloid Polypeptide

Figure 9

The influence of Al(III) on the EGCG-interfered hIAPP fibrillation. (a) Kinetics of 16 μM hIAPP fibrillation without (■) or with 2 μM EGCG interfered by addition of 0 (●), 1 (▲), 2 (▼), 4 (◂), 16 (▸), and 64 (◆) μM Al(III) monitored by ThT fluorescence. The values represent mean (). Solid lines represent the curves fitted using (1). (b) Lag time, (c) elongation rate, and (d) the normalized maximum ThT fluorescence of hIAPP aggregation with 2 μM EGCG on the Al(III) concentration. The intensity values were normalized to hIAPP alone data.

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(b)

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(d)