The Microtubule-Associated Protein Tau and Its Relevance for Pancreatic Beta Cells
Insulin secretion. (a) Insulin secretion after stimulation for 2 h with 20 mM KCl. Insulin secretion from MAPT overexpressing cells versus Empty was calculated in the percentage of total intracellular insulin content; . (b) MAPT overexpressing Rin-5F cell line versus Empty over a time period of 24 hours. Cell lines were either grown in RMPI 1640 medium or in RMPI 1640 medium supplemented with 1 μM Wortmannin. Each column representing relative insulin secretion level is obtained for respective cell line as described in the legend attached directly to the graph. The insulin secretion was normalized to the total intracellular insulin content. Data is presented from 3 independent experiments ± SD.