Methylglyoxal Impairs Insulin Secretion of Pancreatic β-Cells through Increased Production of ROS and Mitochondrial Dysfunction Mediated by Upregulation of UCP2 and MAPKs

Figure 1

Effects of MG on insulin secretion by MIN6 (a) and INS-1 (b) cells. Insulin secretion stimulated by low glucose concentration (5 mM) or high glucose concentration (25 mM) was reduced by preincubation with MG. The effects of MG on MIN6/INS-1 were dependent on the concentration of MG and were reversed by coincubation with NAC. , , and compared to control group (0 mM MG + 0 mM NAC) with 5 mM glucose stimulated. , compared to the higher MG group (0.1 mM MG + 0 mM NAC) with 5 mM glucose stimulated. , compared to control group (0 mM MG + 0 mM NAC) with 25 mM glucose stimulated. compared to the higher MG group (0.1 mM MG + 0 mM NAC) with 25 mM glucose stimulated.

(a)

(b)