Methylglyoxal Impairs Insulin Secretion of Pancreatic β-Cells through Increased Production of ROS and Mitochondrial Dysfunction Mediated by Upregulation of UCP2 and MAPKs

Figure 2

Effects of MG on MIN6 cell apoptosis. Apoptotic rate was higher in MG-treated MIN6 than in controls, and this effect was relieved by coculturing with NAC. (a) Apoptosis was measured by Annexin V-FITC/PI assay, and FITC-positive but PI-negative cells were quantified by flow cytometer. (b) Statistical results of (a). (c) Caspase-3 activity. , compared to control group (0 mM MG + 0 mM NAC). , compared to the higher MG group (0.1 mM MG + 0 mM NAC).

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