Protective Effects of Celastrol on Diabetic Liver Injury via TLR4/MyD88/NF-κB Signaling Pathway in Type 2 Diabetic Rats

Figure 4

Celastrol administration downregulated downstream inflammatory cytokine IL-1β and TNFα expressions in diabetic rats liver. (a-b) Immunohistochemical staining of IL-1β and TNFα (×400). (c-d) Relative mRNA expression levels of IL-1β and TNFα. (e-f) Relative protein expression levels of IL-1β and TNFα were analyzed by Western Blotting, and the ratio of IL-1β/actin and TNFα/actin was shown. (g-h) Serum contents of IL-1β and TNFα. The results are expressed as mean ± SEM, versus NC group; versus DM group; versus CL group; versus CM group.

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