Protective Effects of Celastrol on Diabetic Liver Injury via TLR4/MyD88/NF-κB Signaling Pathway in Type 2 Diabetic Rats

Table 1

Sequences of real-time PCR primers.

Primer name	Sequence	Annealing temperature	Size
TLR4	Forward 5′-ATGAGGACTGGGTGAGAAAC-3′	52°C	161 bp
	Reverse 5′-CACCACCACAATAACTTTCC-3′
MyD88	Forward 5′-TGGTGGTTGTTTCTGACGAT-3′	58.4°C	165 bp
	Reverse 5′-CGCAGATAGTGATGAACCGT-3′
NF-κB	Forward 5′-AAAAACGCATCCCAAGGTGC-3′	52°C	185 bp
	Reverse 5′-AAGCTCAAGCCACCATACCC-3′
IL-1β	Forward 5′-GGACAGAACATAAGCCAACA-3′	61.4°C	127 bp
	Reverse 5′-CTTTCATCACACAGGACAGG-3′
TNFα	Forward 5′-TCCCAGGTTCTCTTCAAGG-3′	61.4°C	177 bp
	Reverse 5′-GTACATGGGCTCATACCAG-3′
GAPDH	Forward 5′-GCAAGTTCAACGGCACAG-3′	52°C	218 bp
	Reverse 5′-GCCAGTAGACTCCACGACAT-3′

PCR: polymerase chain reaction; TLR4: Toll-like receptor 4; MyD88: myeloid differentiation factor 88; NF-κB: nuclear factor-kappa B; IL-1β: interleukin-1 beta; TNFα: tumor necrosis factor alpha; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.