Considerations for Defining Cytokine Dose, Duration, and Milieu That Are Appropriate for Modeling Chronic Low-Grade Inflammation in Type 2 Diabetes
Islet viability and function following overnight treatment with different cytokine concentrations. (a) Cell death measurements by propidium iodide (PI) were made on islets following treatment with Roswell Park Memorial Institute 1640 media (untreated), 10 pg/mL IL-1beta + 20 pg/mL TNF-alpha + 200 pg/mL IFN-gamma (T2D-like), or 500 pg/mL IL-1beta + 1000 pg/mL TNF-alpha + 10,000 pg/mL IFN-gamma (T1D-like). (b) Glucose-stimulated insulin secretion (GSIS) in 3 mM glucose (3G, open bars) and 28 mM glucose (28G, filled bars) following cytokine treatments listed in (a). value < 0.05.
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