A Glyoxalase-1 Knockdown Does Not Have Major Short Term Effects on Energy Expenditure and Atherosclerosis in Mice

Figure 4

(a) En face preparations were performed in order to quantify the amount of atherosclerotic plaques. Bars refer to the mean of oil red O positive areas, analyzed in relation to the total area of the aortic arch ± standard deviation. The knockdown of Glo1 did not show any significant effect on the formation of atherosclerotic plaques (). (b) Transversal sections of the aortic sinus were stained via a MOVAT staining in order to analyze the atherosclerotic plaque formation. Bars represent the mean of the aortic lumina (as determined by ImageJ analysis, lumen area in relation to the total area surrounded by the outer media). The Glo1 knockdown did not have a significant impact of the stenosis caused by atherosclerotic plaques (). (c) Immunohistochemical detection of Glo1 performed with transversal sections of the aortic sinus. Glo1 was mainly detected in the atherosclerotic plaques.

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