ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis

Figure 6

Immunofluorescence staining of HT22 cells to determine the ClC-3 protein expression, stimulated under different glucose concentrations (a), intensity of images, as measured using Image Pro Plus 6.0 (b), and the “average intensity per cell” calculated by dividing the intensity of the image by its total number of cells (c). HT22 cells expressed ClC-3 protein marker. The intensity of the images and the intensity per cell in the high glucose (50 mM) group were significantly higher than those of control and low glucose (30 mM) group ().

(a)

(b)

(c)