ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis

Figure 7

Western blot analysis of HT22 cell lysates from control and low and high glucose groups, in the presence of 0.1 M NPPB, at 24 h, stained for ClC-3 protein expression (a) reveals no change in the total ClC-3 expression, in terms of their intensity, among groups (b). MTT assay of HT22 cells, cultured under same conditions, showed no significant changes in the cell viability among the groups (c and d).

(a)

(b)

(c)

(d)