Potentiation of Calcium Influx and Insulin Secretion in Pancreatic Beta Cell by the Specific TREK-1 Blocker Spadin

Figure 2

Effects of SPA on insulin secretion from isolated islets and MIN6-B1 cells. Mouse islets (a) or MIN6-B1 cells (b) were incubated at 5 mM (Cont) or stimulating concentration of 30 mM KCl in the presence or in the absence of 10⁻⁷ M SPA for 45 min. Mouse islets (c) and MIN6-B1 cells (d) were incubated under basal (2.8 mM glucose) or under stimulating (16.7 mM glucose) conditions in the presence or in the absence of 10⁻⁷ M of SPA for 45 min. The amount of secreted insulin was normalized using the intracellular insulin concentration and was expressed in μg/L. Each value represents the mean ± SEM from 3 independent experiments ( and ).

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